Staff

Dr inż. Katarzyna Juzoń-Sikora

Assistant Professors

Department of Biotechnology

oat × maize addition (OMA) lines • androgenesis • wide crossing of cereals • doubled haploids production • soil drought stress • legumes

(+48) 12 4251833 (wew./ext. 52) k.juzon@ifr-pan.edu.pl

RESEARCH EXPERIENCE

2021 (4 months)	research stay at Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany AS CR
since 2019	assistant professor (adiunkt), Department of Biotechnology, The Franciszek Górski Institute of Plant Physiology PAS
2017 – 2018	research fellowship within Visegrad Scholarship Program, 5 months, Department of Chemical Biology and Genetics, Centre of the Region Hanå for Biotechnological and Agricultural Research, Palacki University Olomouc & Institute of Experimental Botany AS CR
2016 – 2019	technical-scientific position, Department of Cell Biology, The Franciszek Górski Institute of Plant Physiology PAS
2010 – 2015	Ph.D. student, Department of Biotechnology, The Franciszek Górski Institute of Plant Physiology PAS

EDUCATION & ACADEMIC DEGREES

2018 – 2019	postgraduate studies in molecular biology, Faculty of Biochemistry, Biophysics and Biotechnology of the Jagiellonian University
2015	Ph.D. in agricultural sciences, speciality: agronomy, plant physiology, The Franciszek Górski Institute of Plant Physiology PAS
2010 – 2015	Doctoral Studies in Natural Sciences at the Polish Academy of Sciences in Cracow
2010	M.Sc. in Agriculture, University of Agriculture in Krakow
2005 – 2010	Master studies in Agriculture, University of Agriculture in Krakow

RESEARCH INTERESTS

Functioning of oat × maize hybrids
In vitro culture techniques - anthers/microspore cultures
Methods for doubled haploid (DH) production
Physiological factors determining the effectiveness of androgenesis induction
Plants tolerance to soil drought stress

PRACTICAL SKILLS

Fluorescence in situ hybridization (FISH)
The methods of in vitro cultures
Doubled haploids production: androgenesis and wide crossing
Spectrophotometric analysis of phenolic compounds, carbohydrates, proteins, sugars, antioxidant potential
Chromatographic analysis of poliamines (HPLC).

PUBLICATIONS

Juzoń-Sikora K., Laskoś K., Warchoł M., Czyczyło-Mysza I., Dziurka K., Grzesiak M., Skrzypek E.

Water Relations and Physiological Traits Associated with the Yield Components of Winter Wheat (Triticum aestivum L.)

Agriculture, 14: 1887 2024

Laskoś K., Czyczyło-Mysza I., Waligórski P., Dziurka K., Skrzypek E., Warchoł M., Juzoń-Sikora K., Janowiak F., Dziurka ., Grzesiak M., Grzesiak S., Quarrie S., Marcińska I.

Characterising biological and physiological drought signals in diverse parents of a wheat mapping population

International Journal of Molecular Sciences, 25: 6573 2024

Warchoł M., Juzoń-Sikora K., Rančić D., Pećinar I., Warzecha T., Idziak-Helmcke D., Laskoś K., Czyczyło-Mysza I., Dziurka K., Skrzypek E.

Comparative characteristics of oat doubled haploids and oat × maize addition lines: Anatomical features of the leaves, chlorophyll a fluorescence and yield parameters

PLoS ONE, 19: e0298072 2024

Warchoł M., Skrzypek E., Juzoń-Sikora K., Jakovljević D.

Oat (Avena sativa L.) In vitro cultures: prospects and challenges for breeding

Agronomy, 13: 2604 2023

Juzoń-Sikora K., Nowicka A., Plačková L., Doležal K., Żur I.

Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis

Plant Cell, Tissue and Organ Culture, 152: 583–604 2023

Żur I., Adamus A., Cegielska-Taras T., Cichorz S., Dubas E., Gajecka M., Juzoń-Sikora K., Kiełkowska A., Malicka M., Oleszczuk S., Skrzypek E., Szała L., Szarejko I., Zimny J.

Doubled haploids: Contributions of poland’s academies in recognizing the mechanism of gametophyte cell reprogramming and their utilization in breeding of agricultural and vegetable species

Acta Societatis Botanicorum Poloniae, 91: 9128 2022

Laskoś K., Myśków B., Dziurka M., Warchoł M., Dziurka K., Juzoń-Sikora K., Czyczyło-Mysza I.

Variation between glaucous and non‑glaucous near‑isogenic lines of rye (Secale cereale L.) under drought stress

Scientific Reports, 12: 22486 2022

Juzoń-Sikora K., Warchoł M., Dziurka K., Czyczyło-Mysza I., Marcińska I., Skrzypek E.

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

PeerJ: e12854 2022

Dziurka K., Dziurka M., Muszyńska E., Czyczyło-Mysza I., Warchoł M., Juzoń-Sikora K., Laskoś K., Skrzypek E.

Anatomical and hormonal factors determining the development of haploid and zygotic embryos of oat (Avena sativa L.)

Scientific Reports, 12: 548 2022

Skalický V., Vojtková T., Pěnčík A., Vrána J., Juzoń-Sikora K., Koláčková V., Sedlářová M., Kubeš M.F., Novák O.

Auxin metabolite profiling in isolated and intact plant nuclei

International Journal of Molecular Sciences, 22: 12369 2021

Żur I., Kopeć P., Surówka E., Dubas E., Krzewska M., Nowicka A., Janowiak F., Juzoń-Sikora K., Springer A., Barna B., Fodor J.

Impact of ascorbate-glutathione cycle components on the Effectiveness of embryogenesis induction in isolated microspore cultures of barley and triticale

Antioxidants, 10: 1254 2021

Skrzypek E., Warchoł M., Czyczyło-Mysza I., Juzoń-Sikora K., Dziurka K., Marcińska I.

Oat doubled haploid production through wide hybridization with maize

Segui-Simarro J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287: 323-332. Humana, New York, NY. 2021

Warchoł M., Dziurka K., Czyczyło-Mysza I., Juzoń-Sikora K., Marcińska I., Skrzypek E.

Oat (Avena sativa L.) anther culture

Segui-Simarro J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287: 313-322. Humana, New York, NY 2021

Żur I., Dubas E., Krzewska M., Kopeć P., Nowicka A., Surówka E., Gawrońska K., Gołębiowska G., Juzoń-Sikora K., Malaga S.

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Plant Cell Tiss Organ Cult, 145: 347–366 2021

Warchoł M., Juzoń-Sikora K., Dziurka K., Czyczyło-Mysza I., Laskoś K., Marcińska I., Skrzypek E.

The effect of zinc, copper and silver ions on oat (Avena sativa L.) androgenesis

Plants, 10: 248 2021

Juzoń-Sikora K., Idziak-Helmcke D., Rojek-Jelonek M., Warzecha T., Warchoł M., Czyczyło-Mysza I., Dziurka K., Skrzypek E.

Functioning of the photosynthetic apparatus in response to drought stress in Oat × Maize addition lines

International Journal of Molecular Sciences, 21: 6958 2020

Marcińska I., Dziurka K., Waligórski P., Janowiak F., Skrzypek E., Warchoł M., Juzoń-Sikora K., Laskoś K., Czyczyło-Mysza I.

Exogenous polyamines only indirectly induce stress tolerance in wheat growing in hydroponic culture under polyethylene glycol-induced osmotic stress

Life, 10: 151 2020

Zieliński K., Krzewska M., Żur I., Juzoń-Sikora K., Kopeć P., Nowicka A., Moravčiková J., Skrzypek E., Dubas E.

The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cultures of rye (Secale cereale L.)

Plant Cell, Tissue and Organ Culture, 140: 577–592 2020

Dubas E., Zieliński K., Krzewska M., Żur I., Nowicka A., Juzoń-Sikora K.

Identyfikacja czynników determinujących efektywność otrzymywania podwojonych haploidów żyta (Secale cereale L.) metodami androgenezy i krzyżowań oddalonych

Biuletyn IHAR, 286: 135-137 2019

Bączek-Kwinta R., Juzoń-Sikora K., Borek M., Antonkiewicz J.

Photosynthetic response of cabbage in cadmium-spiked soil

Photosynthetica, 57: 731-739 2019

Juzoń-Sikora K., Czyczyło-Mysza I., Ostrowska A., Marcińska I., Skrzypek E.

Chlorophyll fluorescence for prediction of yellow lupin (Lupinus luteus L.) and pea (Pisum sativum L.) susceptibility to drought

Photosynthetica, 57: 950-959 2019

Nowicka A., Juzoń-Sikora K., Krzewska M., Dziurka M., Dubas E., Kopeć P., Zieliński K., Żur I.

Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale

Plant Science, 287: 110189 2019

Juzoń-Sikora K., Czyczyło-Mysza I., Marcińska I., Dziurka M., Waligórski P., Skrzypek E.

Polyamines in yellow lupin (Lupinus luteus L.) tolerance to soil drought

Acta Physiologiae Plantarum, 39: 202 2017

Noga A., Skrzypek E., Warchoł M., Czyczyło-Mysza I., Dziurka K., Marcińska I., Juzoń-Sikora K., Warzecha T., Sutkowska A., Nita Z., Werwińska K.

Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media

In Vitro Cellular & Developmental Biology - Plant, 52: 590-597 2016

Skrzypek E., Warchoł M., Czyczyło-Mysza I., Marcińska I., Nowakowska A., Dziurka K., Juzoń-Sikora K., Noga A.

The effect of light intensity on the production of oat (Avena sativa L.) doubled haploids through oat × maize crosses

Cereal Research Communications, 44: 490-500 2016

Warchoł M., Skrzypek E., Nowakowska A., Marcińska I., Czyczyło-Mysza I., Dziurka K., Juzoń-Sikora K., Cyganek K.

The effect of auxin and genotype on the production of Avena sativa L. doubled haploids lines

Plant Growth Regulation, 78: 155-165 2016

Nowakowska A., Skrzypek E., Marcińska I., Czyczyło-Mysza I., Dziurka K., Juzoń-Sikora K., Cyganek K., Warchoł M.

Application of chosen factors in the wide crossing method for the production of oat double haploids

Open Life Sciences, 10: 112-118 2015

Czyczyło-Mysza I., Marcińska I., Skrzypek E., Cyganek K., Juzoń-Sikora K., Karbarz M.

QTL mapping for germination of seeds obtained from previous wheat generation under drought

Central European Journal of Biology, 9: 374-382 2014

Marcińska I., Czyczyło-Mysza I., Skrzypek E., Grzesiak M., Janowiak F., Filek M., Dziurka M., Dziurka K., Waligórski P., Juzoń-Sikora K., Cyganek K., Grzesiak S.

Alleviation of osmotic stress effects by exogenous application of salicylic or abscisic acid on wheat seedlings

International Journal of Molecular Sciences, 14: 13171-13193 2013

Juzoń-Sikora K., Skrzypek E., Czyczyło-Mysza I., Marcińska I.

Effect of soil drought on the yield structure, protein and phenolics content in Pisum sativum and Lupinus luteus

Acta Agronomica Hungarica, 61: 267-278 2013

Juzoń-Sikora K., Skrzypek E., Marcińska I., Czyczyło-Mysza I.

Zmiany zawartości osmoprotektantów w liściach grochu siewnego i łubinu żółtego w warunkach suszy glebowej

Episteme: Czasopismo Naukowo-Kulturalne, 18: 169-177 2013

Juzoń-Sikora K., Marcińska I., Skrzypek E.

Zmiany wartości żywieniowej roślin bobowatych w warunkach suszy glebowej

Episteme Czasopismo Naukowo-Kulturalne 15: 113-119 2012

Juzoń-Sikora K., Skrzypek E., Ostrowska A., Czyczyło-Mysza I., Marcińska I.

Zmiany zawartości białek rozpuszczalnych i związków fenolowych w liściach grochu siewnego i łubinu żółtego w warunkach suszy glebowej

W: Biomeditech. Badania i Innowacje, Zeszyty Naukowe. Czubenko M., Merta T. (red.), Politechnika Gdańska, Gdańsk, 2011: 33-39 2011

Projects

Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.)

Project number: MRiRW nr 4

Financing: MRiRW

Year of launch: 2021

Year of completion: 2026

Kierownicy projektu: I. Żur

Contractors: E. Dubas, K. Juzoń-Sikora, P. Kopeć, M. Krzewska, A. Nowicka, E. Surówka, P. Waligórski, Weigt D., Mikołajczyk S.

Określenie fizjologicznych i biochemicznych wskaźników tolerancji pszenicy ozimej (Triticum aestivum L.) na stres suszy i wysokiej temperatury

Project number: MRiRW nr 3

Financing: MRiRW

Year of launch: 2021

Year of completion: 2026

Kierownicy projektu: K. Juzoń-Sikora

Contractors: E. Skrzypek, I. Marcińska, I. Czyczyło-Mysza, M. Warchoł, K. Dziurka, A. Ostrowska, K. Laskoś

Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.)

Project number: 2015/18/M/NZ3/00348

Financing: National Science Centre

Year of launch: 2016

Year of completion: 2021

Kierownicy projektu: I. Żur

Contractors: E. Dubas, F. Janowiak, M. Krzewska, A. Nowicka, E. Surówka, S. Malaga, K. Zieliński, Jochen Kumlehn, Balazs Barna, Jozsef Fodor, K. Juzoń-Sikora

Project number: 2020/04/X/NZ2/00396 MINIATURA

Financing:

Year of launch: 2020

Year of completion: 2021

Kierownicy projektu: K. Juzoń-Sikora

Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.)

Project number: Tango2/340285/NCBR/2017

Financing: National Centre of Research and Development

Year of launch: 2017

Year of completion: 2020

Kierownicy projektu: I. Żur

Contractors: E. Dubas, M. Krzewska, K. Juzoń-Sikora, Maria Wędzony

Obtaining of oat doubled haploid lines by wide crossing method, and the identification of partial hybrids

Project number: PBS3/B8/17/2015

Financing: National Centre of Researc and Development

Year of launch: 2015

Year of completion: 2018

Kierownicy projektu: E. Skrzypek

Contractors: I. Marcińska, I. Czyczyło-Mysza, K. Dziurka, M. Warchoł, K. Juzoń-Sikora

Development of an efficient method of oat doubled haploid production

Project number: HOR hn-801-1/13

Financing: Ministry Of Agriculture And Rural Development, The Department of Plant Breeding and Protection

Year of launch: 2013

Year of completion: 2013

Kierownicy projektu: E. Skrzypek

Contractors: I. Czyczyło-Mysza, I. Marcińska, K. Juzoń-Sikora

Determination of polyamines and other osmoprotectants in stimulation of resistance of wheat to osmotic stress

Project number: 480/N-COST/2009/0

Financing: Ministry Of Agriculture And Rural Development, Action COST FA0605 (INPAS

Year of launch: 2009

Year of completion: 2011

Kierownicy projektu: I. Marcińska

Contractors: E. Skrzypek, I. Czyczyło-Mysza, S. Grzesiak, M. Filek, J. Biesaga-Kościelniak, T. Hura, F. Janowiak, P. Waligórski, A. Nowakowska, K. Juzoń-Sikora

Staff

Dr inż. Katarzyna Juzoń-Sikora

research stay at Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany AS CR

Water Relations and Physiological Traits Associated with the Yield Components of Winter Wheat (Triticum aestivum L.)

Characterising biological and physiological drought signals in diverse parents of a wheat mapping population

Comparative characteristics of oat doubled haploids and oat × maize addition lines: Anatomical features of the leaves, chlorophyll a fluorescence and yield parameters

Oat (Avena sativa L.) In vitro cultures: prospects and challenges for breeding

Hormonal homeostasis associated with effective induction of triticale microspore embryogenesis

Doubled haploids: Contributions of poland’s academies in recognizing the mechanism of gametophyte cell reprogramming and their utilization in breeding of agricultural and vegetable species

Variation between glaucous and non‑glaucous near‑isogenic lines of rye (Secale cereale L.) under drought stress

The effect of 2,4-dichlorophenoxyacetic acid on the production of oat (Avena sativa L.) doubled haploid lines through wide hybridization

Anatomical and hormonal factors determining the development of haploid and zygotic embryos of oat (Avena sativa L.)

Auxin metabolite profiling in isolated and intact plant nuclei

Impact of ascorbate-glutathione cycle components on the Effectiveness of embryogenesis induction in isolated microspore cultures of barley and triticale

Oat doubled haploid production through wide hybridization with maize

Oat (Avena sativa L.) anther culture

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

The effect of zinc, copper and silver ions on oat (Avena sativa L.) androgenesis

Functioning of the photosynthetic apparatus in response to drought stress in Oat × Maize addition lines

Exogenous polyamines only indirectly induce stress tolerance in wheat growing in hydroponic culture under polyethylene glycol-induced osmotic stress

The effect of glutathione and mannitol on androgenesis in anther and isolated microspore cultures of rye (Secale cereale L.)

Identyfikacja czynników determinujących efektywność otrzymywania podwojonych haploidów żyta (Secale cereale L.) metodami androgenezy i krzyżowań oddalonych

Photosynthetic response of cabbage in cadmium-spiked soil

Chlorophyll fluorescence for prediction of yellow lupin (Lupinus luteus L.) and pea (Pisum sativum L.) susceptibility to drought

Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale

Polyamines in yellow lupin (Lupinus luteus L.) tolerance to soil drought

Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media

The effect of light intensity on the production of oat (Avena sativa L.) doubled haploids through oat × maize crosses

The effect of auxin and genotype on the production of Avena sativa L. doubled haploids lines

Application of chosen factors in the wide crossing method for the production of oat double haploids

QTL mapping for germination of seeds obtained from previous wheat generation under drought

Alleviation of osmotic stress effects by exogenous application of salicylic or abscisic acid on wheat seedlings

Effect of soil drought on the yield structure, protein and phenolics content in Pisum sativum and Lupinus luteus

Zmiany zawartości osmoprotektantów w liściach grochu siewnego i łubinu żółtego w warunkach suszy glebowej

Zmiany wartości żywieniowej roślin bobowatych w warunkach suszy glebowej

Zmiany zawartości białek rozpuszczalnych i związków fenolowych w liściach grochu siewnego i łubinu żółtego w warunkach suszy glebowej

Projects

Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.)

Określenie fizjologicznych i biochemicznych wskaźników tolerancji pszenicy ozimej (Triticum aestivum L.) na stres suszy i wysokiej temperatury

Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.)

Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.)

Obtaining of oat doubled haploid lines by wide crossing method, and the identification of partial hybrids

Development of an efficient method of oat doubled haploid production

Determination of polyamines and other osmoprotectants in stimulation of resistance of wheat to osmotic stress